The scientific foundation behind ORYL F1 — combining second harmonic scattering and linear light scattering for deep molecular insight.
Technology
Ultrafast Light Scattering
What is Ultrafast Light Scattering?
Ultrafast Light Scattering (ULS) is the category name for the measurement technology at the core of the ORYL F1. It combines two complementary optical techniques — second harmonic scattering (SHS) and linear light scattering (LLS) — into a single, high-throughput platform.
This dual-readout approach provides molecular-level insight into solubility and aggregation behavior that cannot be obtained from either technique alone. ULS enables scientists to detect subtle changes — including early-onset aggregation at the nanometer scale — well before they become visible through conventional turbidity measurements.
Complementary Readouts
Two techniques, one platform
Second Harmonic Scattering (SHS)
SHS is a nonlinear optical technique that is sensitive to molecular-level organization and symmetry breaking at interfaces. In the context of solubility and aggregation measurement, SHS probes early-onset aggregation events — changes that occur at the nanometer scale before particles grow large enough to scatter light in the conventional sense.
This makes SHS a powerful complement to LLS: it reveals the earliest stages of aggregation, providing insight that precedes turbidity-based detection by a significant margin.
Linear Light Scattering (LLS)
LLS is a well-established technique for characterizing particle size and scattering behavior. In the ORYL F1, LLS provides a complementary readout to SHS — tracking how particles evolve in size as aggregation progresses.
Together, SHS and LLS offer a complete picture of solubility and aggregation: from molecular-level onset (SHS) through particle growth (LLS). This dual measurement approach delivers robust and reliable results that support confident decision-making.
Advantages
Why Ultrafast Light Scattering matters
Earlier detection
Detect early onset of aggregation prior to turbidity. SHS reveals nanometer-scale changes that conventional light scattering methods miss entirely.
Higher throughput
Profile a full 384-well plate in approximately 15 minutes. Roughly 100× higher throughput compared to traditional HPLC-based solubility workflows.
Lower compound consumption
Use approximately 100× less compound than HPLC-based methods. Practical for early-stage work where compound is scarce.
Complementary readouts
Dual SHS + LLS measurement provides a more complete picture of solubility and aggregation than either technique alone.
Scientific Publications & Posters
Explore peer-reviewed publications and conference posters on ULS and its applications.
Want to see ULS data from your own compounds?
Request a demo or send samples for a measurement service.